Sequence of a giardin subunit cDNA fromGiardia lamblia

Sequence of a giardin subunit cDNA from Giardia lamblia.

The filamentous protein giardin is structurally associated with microtubules in Giardia lamblia. We present the sequence of one giardin chain (beta-giardin) corresponding to band 14B of Giardia cytoskeleton proteins (1). A Giardia cDNA library cloned and expressed in lamda gt11 was screened with antibodies monospecific to giardin (2). One positive phage clone (SR7) selected for sequencing conta...

Giardia lamblia: intracellular localization of alpha8-giardin.

Alpha8-giardin (α8-giardin) is a member of the multi-gene α-giardin family in the intestinal parasitic protozoan, Giardia lamblia. This gene family shares an ancestry with the annexin super family, whose common characteristic is calcium dependent binding to membranes that contain acidic phospholipids. In the present study, the antigenicity, hydrophilicity, flexibility, surface probability, and ...

cDNA sequence of an apple ribulose bisphosphate carboxylase small subunit.

Apo and calcium-bound crystal structures of Alpha-11 giardin, an unusual annexin from Giardia lamblia.

Alpha-11 giardin is a member of the multi-gene alpha-giardin family in the intestinal protozoan, Giardia lamblia. This gene family shares an ancestry with the annexin super family, whose common characteristic is calcium-dependent binding to membranes that contain acidic phospholipids. Several alpha giardins are highly expressed during parasite-induced diarrhea in humans. Despite being a member ...

Antibodies to the ventral disc protein delta-giardin prevent in vitro binding of Giardia lamblia trophozoites.

A cDNA coding for detlaq-giardin was cloned from Giardia lamblia trophozoites to localize the protein and to study its function in mediating surface attachment. Recombinant delta-giardin antigen was expressed in Escherichia coli as a poly-histidine fusion protein and was purified by affinity chromatography for production of antisera to delta-giardin. By immunoblotting analysis, antisera to reco...